Mouse Lipopolysaccharides(LPS) ELISA Kit

Product Details

Customer Reviews and Q&A

Name: Mouse Lipopolysaccharides(LPS) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13066m

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Code: CSB-E13066m

Rate （5.0）
Review （1）
Citation （163）

Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.156 ng/mL-10 ng/mL

Sensitivity: 0.039 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

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Product Details
Customer Reviews and Q&A
Target Background

Abbreviation

LPS

Species

Mus musculus (Mouse)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	98
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	96
	Range %	86-98

Recovery

The recovery of mouse LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	94	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.424	2.522	2.473	2.406
5	2.051	1.955	2.003	1.936
2.5	1.345	1.255	1.300	1.233
1.25	0.679	0.655	0.667	0.600
0.625	0.461	0.444	0.453	0.386
0.312	0.257	0.239	0.248	0.181
0.156	0.171	0.165	0.168	0.101
0	0.069	0.065	0.067

Protocols

/uploadfile/Ins/CSB-E13066m.pdf

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

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Mouse Lipopolysaccharides(LPS) ELISA Kit

Description

This Mouse LPS ELISA Kit was designed for the quantitative measurement of Mouse LPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Sample species : a:1:{i:0;a:2:{i:0;s:5:"Mouse";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:22:"Plasma (anticoagulant)";i:1;N;}}

Review: I used the Mouse Lipopolysaccharide ELISA Kit (CSB-E13066m) for quantification of LPS in plasma samples from aged vs. young and aged+hALA vs. aged+lALA. Their ODs range from 0.37 to 1.773.

By Anonymous

■ Q&A

Hi, i have some questions. i am interested about CSB-E13066m elisa kit.its sample types showed to serum, plasme etc. i want serum ascites type

Thanks for your inquiry.
Serum and serum ascites are 2 different sample types.Serum is more normal,and you can refer the information in the published papers at the bottom of the product page.

If you want to detect serum ascites,we suggest you do pre-testing first.Pls let me know if you have any further questions. Thank you.

When using Mouse Lipopolysaccharides(LPS) ELISA Kit, can I finally translate the results (ng/ml) into the EU/ml?
what is the EU/ml of the standard provided?

Thanks for your inquiry.The the results (ng/ml) can not be translated into the EU/ml results as there is no reduction formula between them.
This kit is supplied with 2 vial of frozen dry standard and the concentration is 10 ng/mL after dissolution;Pls let me know if you have any further questions. Thank you.

I am curious what product you would recommend to detect mouse lipopolysaccharides with very small volumes (less than 10 uL)

Thanks for your inquiry. The amount is too small, if detected, false negatives may exist.Pls consider it carefully.Pls let me know if you have any further questions. Thank you.

Please provide the following information regarding Mouse Lipopolysaccharides, LPS ELISA Kit (CSB-E13066m)
Can customer use diluent buffer to dilute their plasma samples?

Thanks for your inquiry.
CSB-E13066m
The reagent system of this kit is very special, and dilution is not needed if you test serum or plasma samples.
If you confirm that dilution is needed after the pretest, we suggest you use sample diluent. About pretest pls refer to: https://www.cusabio.com/m-226.html
Pls let me know if you have any further questions. Thank you.