CSF1

Colony-stimulating factor 1 (CSF1), also commonly known as macrophage colony-stimulating factor (M-CSF), is a glycoprotein. It binds to the CSF1 receptor (CSF1R) on the cell surface to activate multiple downstream signaling pathways, such as the PI3K/Akt and Ras/MAPK pathways. These actions promote the survival, proliferation, and differentiation of monocytes, macrophages, and their precursors.
Aberrant CSF1 signaling is linked to various diseases, including cancers, where it can contribute to tumor - associated macrophage recruitment and tumor progression, as well as certain inflammatory and neurodegenerative disorders. In drug development, CSF1R inhibitors are being actively explored to target the CSF1 pathway, aiming to disrupt the tumor microenvironment and treat related diseases.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of THP-1 cells is 2.880-4.115 ng/mL.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CSF1 protein at 2 μg/ml can bind Anti-CSF1 recombinant antibody (CSB-RA006043MA1HU). The EC₅₀ is 4.291-4.988 ng/mL.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CSF1 protein at 2 μg/ml can bind Human CSF1R protein (CSB-MP006044HU). The EC₅₀ is 37.37-43.34 ng/mL.

CSF1 Recombinant Monoclonal Antibody (CSB-RA006043MA1HU) captured on Protein A Chip can bind Recombinant Human CSF1 protein with an affinity constant of 0.13 nM as detected by MetaSPR Assay (WeSPRTM 200).

Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial (Active)(CSB-YP006043HU1)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of THP-1 cells is 0.5913-1.205 ng/mL.

The purity of CSF1 was greater than 90% as determined by SEC-HPLC

Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial (Active)(CSB-MP006043HU1)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of THP-1 cells is 2.880-4.115 ng/mL.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CSF1 protein at 2 μg/ml can bind Human CSF1R protein (CSB-MP006044HU). The EC₅₀ is 37.37-43.34 ng/mL.

Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial (Active)(CSB-MP006043MO)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Mouse Csf1 at 2 μg/ml can bind Mouse Csf1r(CSB-MP006044MO). The EC₅₀ is 3.226-4.264 ng/mL.

The purity of Csf1 was greater than 90% as determined by SEC-HPLC

Recombinant Human Macrophage colony-stimulating factor 1 protein (CSF1),Partial (Active)(CSB-AP002131HU)

Validated Data

CSF1 Antibody(CSB-PA06859A0Rb)

Validated Data

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA06859A0Rb at dilution of 1:100

Immunofluorescent analysis of A549 cells using CSB-PA06859A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Human Macrophage Colony-Stimulating Factor,M-CSF ELISA kit(CSB-E04658h)

Validated Data

Code: CSB-E04658h

Size: ,96T,5×96T,10×96T

Sensitivity: 0.039 ng/ml

Detection Range: 0.156 ng/ml - 10 ng/ml

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following CSF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CSF1 Antibodies

CSF1 Proteins

CSF1 ELISA Kits

CSF1 Antibodies

CSF1 Antibodies for Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)

Code	Product Name	Species Reactivity	Application
CSB-PA765584XA01DOT	CSF1 Antibody	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)	ELISA, WB (ensure identification of antigen)

CSF1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA613201XA01SVG	CSF1 Antibody	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ELISA, WB (ensure identification of antigen)

CSF1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA06859A0Rb	CSF1 Antibody	Human	ELISA, IHC, IF
CSB-PA006115	CSF1 Antibody	Human	WB, ELISA
CSB-RA006043MB1HU	CSF1 (Lacnotuzumab Biosimilar) Recombinant Monoclonal Antibody	Human
CSB-RA006043MA1HU	CSF1 Recombinant Monoclonal Antibody	Human	ELISA

CSF1 Antibodies for Mus musculus (Mouse)

Code	Product Name	Species Reactivity	Application
CSB-PA07777A0Rb	Csf1 Antibody	Mouse	ELISA
CSB-PA07779A0Rb	Csf1 Antibody	Mouse	ELISA, IHC
CSB-EAP07779	Csf1 Antibody Pair	Human,Mouse,Guinea pig,Bovine,Pig,Dog,Goat	S-ELISA

CSF1 Proteins

CSF1 Proteins for Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)

Code	Product Name	Source
CSB-YP765584DOT CSB-EP765584DOT CSB-BP765584DOT CSB-MP765584DOT CSB-EP765584DOT-B	Recombinant Ashbya gossypii Protein CSF1 (CSF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CSF1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Source
CSB-YP613201SVG CSB-EP613201SVG CSB-BP613201SVG CSB-MP613201SVG CSB-EP613201SVG-B	Recombinant Saccharomyces cerevisiae Protein CSF1 (CSF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CSF1 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-MP6738MOV	Recombinant Macaca fascicularis macrophage colony-stimulating factor 1 isoform X1 (CSF1), partial	Mammalian cell

CSF1 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP810353RA CSB-EP810353RA CSB-BP810353RA CSB-MP810353RA CSB-EP810353RA-B	Recombinant Rat Macrophage colony-stimulating factor 1 (Csf1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP810353RA	Recombinant Rat Macrophage colony-stimulating factor 1 (Csf1), partial	E.coli

CSF1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP006043HU	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1)	Yeast
CSB-AP002131HU	Recombinant Human Macrophage colony-stimulating factor 1 protein (CSF1),Partial (Active)	E.Coli
CSB-CF006043HU	Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP006043HU CSB-EP006043HU CSB-BP006043HU CSB-MP006043HU CSB-EP006043HU-B	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-AP003621HU	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial (Active)	Mammalian cell
CSB-YP006043HU1	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial (Active)	Yeast
CSB-EP006043HU	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial	E.coli
CSB-MP006043HU1	Recombinant Human Macrophage colony-stimulating factor 1 (CSF1), partial (Active)	Mammalian cell

CSF1 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP006043MO CSB-EP006043MO CSB-BP006043MO CSB-MP006043MO CSB-EP006043MO-B	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-AP003461MO	Recombinant Mouse Macrophage colony-stimulating factor 1 protein (Csf1), partial (Active)	E.Coli
CSB-CF006043MO(A4)	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP006043MO	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	E.coli
CSB-AP003641MO	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial (Active)	Mammalian cell
CSB-YP006043MO	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	Yeast
CSB-YP006043MOd7	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	Yeast
CSB-YP006043MO1	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	Yeast
CSB-EP006043MO1	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial	E.coli
CSB-MP006043MO	Recombinant Mouse Macrophage colony-stimulating factor 1 (Csf1), partial (Active)	Mammalian cell