CTBP1, also known as C-terminal binding protein 1, is a well - studied protein. It has common aliases such as CtBP. Functionally, it acts as a transcriptional corepressor, binding to the C - terminus of adenovirus E1A proteins. In signaling pathways, CTBP1 is involved in the regulation of Notch and Wnt pathways.
Regarding diseases, CTBP1 is implicated in tumorigenesis and cancer progression, with roles in promoting cell survival and metastasis. In drug development, researchers are exploring inhibitors targeting CTBP1 to block its function. These efforts aim to develop new anti - cancer therapies, although significant challenges remain in clinical translation.
Popular
Product:
CTBP1 Recombinant Monoclonal Antibody(CSB-RA546965A0HU)
Validated Data
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg)
All lanes: CtBP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time：2min
IHC image of CSB-RA546965A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA546965A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HepG2 cells stained with CSB-RA546965A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
CTBP1 Antibody(CSB-PA618772ESR1HU)
Validated Data
Western blot
All lanes: CTBP1 antibody at 6µg/ml + A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48, 47 kDa
Observed band size: 48 kDa
The following CTBP1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
