The protein encoded by DDX3X is DEAD-box helicase 3 X-linked, commonly aliased as DDX3, DBX, or DDX31. As an ATP-dependent RNA helicase, it mediates critical steps in RNA metabolism—transcription, splicing, mRNA export, and translation initiation—while regulating cell cycle progression and apoptosis via signaling cascade modulation.
DDX3X intersects with Wnt/β-catenin, PI3K/Akt, and NF-κB pathways, linking it to X-linked intellectual disability, cancers (lung, breast, melanoma), and viral infections (HIV, HCV). Preclinical studies of small-molecule inhibitors like RK-33 show tumor growth suppression by targeting DDX3X, positioning it as a promising therapeutic target.
Popular
Product:
DDX3X Antibody(CSB-PA006621LA01HU)
Validated Data
Western Blot
Positive WB detected in:293 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes:DDX3 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
Immunohistochemistry analysis of human adrenal gland tissue using CSB-PA006621LA01HU at dilution of 1:100
Immunoprecipitating DDX3X in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006621LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006621LA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
Immunofluorescence staining of HepG2 cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following DDX3X
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
