EEF1A1

Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), also known as eEF1A1 or EF-1α, is a key GTPase in protein synthesis. It mediates aminoacyl-tRNA delivery to the ribosomal A-site during translation elongation, ensuring efficient peptide chain extension. Beyond translation, it regulates cytoskeletal dynamics via actin/microtubule interactions and modulates cell signaling cascades.
EEF1A1 overexpression correlates with aggressive phenotypes in breast, lung, and colorectal cancers, driving proliferation and metastasis. Preclinical research explores small-molecule inhibitors targeting its GTPase activity or non-translational functions as potential anticancer agents, though no candidates have entered clinical trials to date.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

EEF1A1 Recombinant Monoclonal Antibody(CSB-RA019747A0HU)

Validated Data

Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg),U-937 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg),THP-1 whole cell lysate(30µg),NIH/3T3 whole cell lysate(30µg),HEK293 whole cell lysate(30µg), Rat brain tissue lysate(30µg)
All lanes: eEF1A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
Exposure time：20s

Immunofluorescence staining of HepG2 cell with CSB-RA019747A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing jurkat cells stained with CSB-RA019747A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

EEF1A1 Antibody(CSB-PA007409LA01HU)

Validated Data

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA007409LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA007409LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

The following EEF1A1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

EEF1A1 Antibodies

EEF1A1 Proteins

EEF1A1 Antibodies

EEF1A1 Antibodies for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Code	Product Name	Species Reactivity	Application
CSB-PA007409ZA01DXU	EEF1A1 Antibody	Cricetulus griseus	ELISA, WB (ensure identification of antigen)

EEF1A1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA007409GA01HU	EEF1A1 Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA000115	Acetyl-EEF1A1 (K41) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA005918	EEF1A1/EEF1A2/EEF1A1P5 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA007409LA01HU	EEF1A1 Antibody	Human	ELISA, IHC, IF
CSB-PA007409LB01HU	EEF1A1 Antibody, HRP conjugated	Human	ELISA
CSB-PA007409LC01HU	EEF1A1 Antibody, FITC conjugated	Human
CSB-PA007409LD01HU	EEF1A1 Antibody, Biotin conjugated	Human	ELISA
CSB-RA019747A0HU	EEF1A1 Recombinant Monoclonal Antibody	Human, Mouse, Rat	ELISA, WB, IF, FC

EEF1A1 Proteins

EEF1A1 Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-YP722246EQV CSB-EP722246EQV CSB-BP722246EQV CSB-MP722246EQV CSB-EP722246EQV-B	Recombinant Pan troglodytes Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Code	Product Name	Source
CSB-YP007409DXU CSB-EP007409DXU CSB-BP007409DXU CSB-MP007409DXU CSB-EP007409DXU-B	Recombinant Cricetulus griseus Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP007409DXU	Recombinant Cricetulus griseus Elongation factor 1-alpha 1 (EEF1A1)	E.coli
CSB-YP007409DXU	Recombinant Cricetulus griseus Elongation factor 1-alpha 1 (EEF1A1)	Yeast

EEF1A1 Proteins for Equus caballus (Horse)

Code	Product Name	Source
CSB-YP007409HO CSB-EP007409HO CSB-BP007409HO CSB-MP007409HO CSB-EP007409HO-B	Recombinant Horse Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Felis catus (Cat) (Felis silvestris catus)

Code	Product Name	Source
CSB-YP721103CA CSB-EP721103CA CSB-BP721103CA CSB-MP721103CA CSB-EP721103CA-B	Recombinant Cat Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP007409RA CSB-EP007409RA CSB-BP007409RA CSB-MP007409RA CSB-EP007409RA-B	Recombinant Rat Elongation factor 1-alpha 1 (Eef1a1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP007409HU CSB-EP007409HU CSB-BP007409HU CSB-MP007409HU CSB-EP007409HU-B	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP007409HU	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)	E.coli
CSB-EP007409HUe1	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)	E.coli
CSB-EP007409HUa0	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)	E.coli
CSB-EP007409HU(M1)	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)(K313R)	E.coli
CSB-EP007409HU(M)	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)(C31A)	E.coli
CSB-EP007409HU(M)c7	Recombinant Human Elongation factor 1-alpha 1 (EEF1A1)(C31A)	E.coli

EEF1A1 Proteins for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Source
CSB-YP007409RB CSB-EP007409RB CSB-BP007409RB CSB-MP007409RB CSB-EP007409RB-B	Recombinant Rabbit Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP007409BO CSB-EP007409BO CSB-BP007409BO CSB-MP007409BO CSB-EP007409BO-B	Recombinant Bovine Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

EEF1A1 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP007409MO CSB-EP007409MO CSB-BP007409MO CSB-MP007409MO CSB-EP007409MO-B	Recombinant Mouse Elongation factor 1-alpha 1 (Eef1a1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP007409MO1	Recombinant Mouse Elongation factor 1-alpha 1 (Eef1a1), partial	E.coli
CSB-EP007409MO2	Recombinant Mouse Elongation factor 1-alpha 1 (Eef1a1), partial	E.coli

EEF1A1 Proteins for Pongo abelii (Sumatran orangutan)

Code	Product Name	Source
CSB-YP725258PYX CSB-EP725258PYX CSB-BP725258PYX CSB-MP725258PYX CSB-EP725258PYX-B	Recombinant Pongo abelii Elongation factor 1-alpha 1 (EEF1A1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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EEF1A1

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