FGF1, also known as Fibroblast Growth Factor 1, is commonly referred to as acidic FGF. It functions by binding to fibroblast growth factor receptors (FGFRs), leading to receptor dimerization and activation of downstream signaling cascades. The major pathways include the Ras-MAPK and PI3K-AKT pathways, which regulate cell proliferation, differentiation, and survival.
Aberrant FGF1 signaling has been linked to various diseases, including cancer, cardiovascular disorders, and neurodegenerative diseases. In drug development, targeting FGF1 or its receptors is emerging as a promising strategy. Some small - molecule inhibitors and monoclonal antibodies are in pre - clinical and clinical trials to modulate its activity for therapeutic benefits.
Popular
Product:
FGF1 Recombinant Monoclonal Antibody(CSB-RA008615MA1HU)
Validated Data
Immunofluorescence staining of U251 cell with CSB-RA008615MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay Peak curve showing PC-3 cells stained with CSB-RA008615MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
FGF1 Antibody(CSB-PA008615ESR1HU)
Validated Data
Western blot
All lanes: Fibroblast growth factor 1 antibody at 6μg/ml
Lane 1: Mouse kidney tissue
Lane 2: Mouse heart tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18, 7 kDa
Observed band size: 18 kDa
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008615ESR1HU at dilution of 1:100
IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA008615ESR1HU diluted at 1:289 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CSB-PA008615ESR1HU at 1:96, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following FGF1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
FGF1 Proteins
FGF1 Proteins for Mesocricetus auratus (Golden hamster)
FGF1 Proteins for Gallus gallus (Chicken)
FGF1 Proteins for Homo sapiens (Human)
FGF1 Proteins for Sus scrofa (Pig)
FGF1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
FGF1 Proteins for Bos taurus (Bovine)
FGF1 Proteins for Xenopus laevis (African clawed frog)
FGF1 Proteins for Cynops pyrrhogaster (Japanese fire-bellied newt)
FGF1 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
FGF1 Proteins for Ovis aries (Sheep)
FGF1 Proteins for Notophthalmus viridescens (Eastern newt) (Triturus viridescens)
FGF1 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
FGF1 Proteins for Rattus norvegicus (Rat)
FGF1 Proteins for Capreolus capreolus (Roe deer)
FGF1 Proteins for Mus musculus (Mouse)
