GHR (Growth Hormone Receptor) is a transmembrane cytokine receptor superfamily member, commonly aliased as GHBP (soluble extracellular domain). It triggers biological effects of growth hormone (GH) via dimerization upon GH binding, activating Janus kinases (JAKs) to initiate intracellular signaling.
GHR signaling involves JAK2-STAT5, MAPK, and PI3K-AKT pathways, regulating growth and metabolism. Mutations cause Laron syndrome (GH insensitivity). Pegvisomant, a GH analog blocking GHR, is an approved drug for acromegaly, inhibiting excessive GH-mediated tissue overgrowth.
Popular
Product:
Recombinant Human Growth hormone receptor (GHR), partial (Active)(CSB-MP009411HU)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized GH1 (CSB-MP009407HU) at 1 μg/ml can bind human GHR, the EC50 of human GHR protein is 24.96-33.39 ng/ml.
Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay.
The purity of GHR was greater than 95% as determined by SEC-HPLC
GHR Recombinant Monoclonal Antibody(CSB-RA978909A0HU)
Validated Data
IHC image of CSB-RA978909A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA978909A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 Cells with CSB-RA978909A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The following GHR
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
GHR Proteins
GHR Proteins for Saimiri boliviensis boliviensis (Bolivian squirrel monkey)
GHR Proteins for Gallus gallus (Chicken)
GHR Proteins for Homo sapiens (Human)
GHR Proteins for Sus scrofa (Pig)
GHR Proteins for Macaca mulatta (Rhesus macaque)
GHR Proteins for Bos indicus (Zebu)
GHR Proteins for Bos taurus (Bovine)
GHR Proteins for Papio anubis (Olive baboon)
GHR Proteins for Cavia porcellus (Guinea pig)
GHR Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
GHR Proteins for Ailuropoda melanoleuca (Giant panda)
GHR Proteins for Ovis aries (Sheep)
GHR Proteins for Rattus norvegicus (Rat)
GHR Proteins for Oryctolagus cuniculus (Rabbit)
GHR Proteins for Columba livia (Rock dove)
GHR Proteins for Mus musculus (Mouse)
