HIF1A

HIF1A, hypoxia-inducible factor 1-alpha, is a transcription factor that is activated under oxygen-deprived conditions in human and mammalian cells. It governs a range of gene expressions, impacting cellular metabolism, proliferation, and survival, thus playing a critical role in cellular adaptation to hypoxia and fostering angiogenesis. HIF1A is vital for embryonic development, tissue homeostasis, and the response to hypoxic stress. Its dysregulation is implicated in pathological processes such as tumorigenesis, inflammation, and immune disorders, making it a significant factor in both physiological and pathological process.

Validated Data

Immunofluorescence staining of Hela cell with CSB-RA624113MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA624113MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,001 events was performed.

Human hypoxia-inducible factor 1α(HIF-1α) ELISA kit(CSB-E12112h)

Validated Data

Code: CSB-E12112h

Size: 96T,5×96T,10×96T

Sensitivity: 15.6 pg/mL

Detection Range: 62.5 pg/mL-4000 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following HIF1A reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

HIF1A Antibodies

HIF1A Proteins

HIF1A ELISA Kits

HIF1A Antibodies

HIF1A Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA010351GA01HU	HIF1A Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA12807A0Rb	HIF1A Antibody	Human	ELISA, IHC, IF
CSB-PA12807B0Rb	HIF1A Antibody, HRP conjugated	Human	ELISA
CSB-PA12807C0Rb	HIF1A Antibody, FITC conjugated	Human
CSB-PA12807D0Rb	HIF1A Antibody, Biotin conjugated	Human	ELISA
CSB-PA930291	HIF1A Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA952643	HIF1A Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA000432	HIF1A Antibody	Mouse,Rat	WB
CSB-PA002906	HIF1A Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA077072	HIF1A Antibody	Human	ELISA,IHC
CSB-PA968396	HIF1A Antibody	Human	ELISA,WB,IHC
CSB-PA928186	HIF1A Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA578243	HIF1A Antibody	Human,Mouse,Rat	ELISA,WB
CSB-RA263943A0HU	HIF1A Recombinant Monoclonal Antibody	Human	ELISA, IF
CSB-RA624113MA1HU	HIF1A Recombinant Monoclonal Antibody	Human	ELISA, IF, FC

HIF1A Proteins

HIF1A Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Code	Product Name	Source
CSB-YP858481OEI CSB-EP858481OEI CSB-BP858481OEI CSB-MP858481OEI CSB-EP858481OEI-B	Recombinant Oncorhynchus mykiss Hypoxia-inducible factor 1-alpha (hif1a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HIF1A Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP896655CH CSB-EP896655CH CSB-BP896655CH CSB-MP896655CH CSB-EP896655CH-B	Recombinant Chicken Hypoxia-inducible factor 1-alpha (HIF1A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HIF1A Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP624113HU CSB-EP624113HU CSB-BP624113HU CSB-MP624113HU CSB-EP624113HU-B	Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP624113HU	Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial	E.coli
CSB-EP624113HU1-B	Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial, Biotinylated	E.coli
CSB-EP624113HU3	Recombinant Human Hypoxia-inducible factor 1-alpha (HIF1A), partial	E.coli

HIF1A Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP010351RA CSB-EP010351RA CSB-BP010351RA CSB-MP010351RA CSB-EP010351RA-B	Recombinant Rat Hypoxia-inducible factor 1-alpha (Hif1a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-MP010351RA1b0	Recombinant Rat Hypoxia-inducible factor 1-alpha（Hif1a), partial	Mammalian cell
CSB-MP010351RA1	Recombinant Rat Hypoxia-inducible factor 1-alpha（Hif1a), partial	Mammalian cell

HIF1A Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP895019BO CSB-EP895019BO CSB-BP895019BO CSB-MP895019BO CSB-EP895019BO-B	Recombinant Bovine Hypoxia-inducible factor 1-alpha (HIF1A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HIF1A Proteins for Bos mutus grunniens (Wild yak) (Bos grunniens)

Code	Product Name	Source
CSB-YP611302BPD CSB-EP611302BPD CSB-BP611302BPD CSB-MP611302BPD CSB-EP611302BPD-B	Recombinant Bos mutus grunniens Hypoxia-inducible factor 1-alpha (HIF1A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HIF1A Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP730731MO CSB-EP730731MO CSB-BP730731MO CSB-MP730731MO CSB-EP730731MO-B	Recombinant Mouse Hypoxia-inducible factor 1-alpha (Hif1a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP730731MO	Recombinant Mouse Hypoxia-inducible factor 1-alpha (Hif1a), partial	Yeast
CSB-MP730731MO	Recombinant Mouse Hypoxia-inducible factor 1-alpha (Hif1a), partial	Mammalian cell
CSB-MP730731MOd7	Recombinant Mouse Hypoxia-inducible factor 1-alpha (Hif1a), partial	Mammalian cell

HIF1A Proteins for Xenopus laevis (African clawed frog)

Code	Product Name	Source
CSB-YP867421XBE CSB-EP867421XBE CSB-BP867421XBE CSB-MP867421XBE CSB-EP867421XBE-B	Recombinant Xenopus laevis Hypoxia-inducible factor 1-alpha (hif1a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

HIF1A Proteins for Eospalax fontanierii baileyi (Plateau zokor) (Eospalax baileyi)

Code	Product Name	Source
CSB-YP653038EAAY CSB-EP653038EAAY CSB-BP653038EAAY CSB-MP653038EAAY CSB-EP653038EAAY-B	Recombinant Eospalax baileyi Hypoxia-inducible factor 1-alpha (HIF1A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli