KRT19

Keratin 19 (KRT19), also called cytokeratin 19 (CK19), belongs to the type I intermediate filament protein family. It forms heterodimers with type II keratins to sustain epithelial cell structural stability, and regulates cell proliferation, migration, and apoptosis via pathways like Wnt/β-catenin and PI3K/Akt.
Abnormal KRT19 expression is associated with cancers including hepatocellular carcinoma (CK19-positive HCC indicates poor prognosis), pancreatic, and breast cancers. Its fragment Cyfra21-1 serves as a clinical tumor biomarker. Preclinical studies are exploring antibody-drug conjugates targeting CK19 for cancer therapy, though no such drugs have entered late-stage trials yet.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

KRT19 Recombinant Monoclonal Antibody(CSB-RA598178A0HU)

Validated Data

Western Blot
Positive WB detected in: MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg)
All lanes: Cytokeratin 19 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 44 kDa
Observed band size: 49 kDa
Exposure time：10s

IHC image of CSB-RA598178A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA598178A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of MCF-7 cell with CSB-RA598178A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing MCF-7 cells stained with CSB-RA598178A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

KRT19 Antibody(CSB-PA165755)

Validated Data

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA165755(KRT19 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-5: A431 cells, Hela cells, Lovo cells, Human fetal lung tissue, Human adult liver tissue, Primary antibody: CSB-PA165755(KRT19 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds

The following KRT19 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

KRT19 Antibodies

KRT19 Proteins

KRT19 ELISA Kits

KRT19 Antibodies

KRT19 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA11259B0Rb	KRT19 Antibody, HRP conjugated	Human	ELISA
CSB-PA11259A0Rb	KRT19 Antibody	Human	ELISA
CSB-PA012534GA01HU	KRT19 Antibody	Human,Mouse,Rat	ELISA,WB,IHC,FC,IF
CSB-PA11259D0Rb	KRT19 Antibody, Biotin conjugated	Human	ELISA
CSB-PA11259C0Rb	KRT19 Antibody, FITC conjugated	Human
CSB-PA100243	KRT19 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA258732	KRT19 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-MA000207	KRT19 Monoclonal Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA002047	KRT19 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA005551	KRT19 Antibody	Human	WB, ELISA
CSB-PA009691	KRT19 Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA165755	KRT19 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA909758	KRT19 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-MA974483	KRT19 Monoclonal Antibody	Human	ELISA, IHC
CSB-RA598178A0HU	KRT19 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC

KRT19 Proteins

KRT19 Proteins for Mesocricetus auratus (Golden hamster)

Code	Product Name	Source
CSB-YP309232MRG CSB-EP309232MRG CSB-BP309232MRG CSB-MP309232MRG CSB-EP309232MRG-B	Recombinant Mesocricetus auratus Keratin, type I cytoskeletal 19 (KRT19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP012534CH CSB-EP012534CH CSB-BP012534CH CSB-MP012534CH CSB-EP012534CH-B	Recombinant Chicken Keratin, type I cytoskeletal 19 (KRT19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-BP012534HU	Recombinant Human Keratin, type I cytoskeletal 19 (KRT19)	Baculovirus
CSB-EP012534HU	Recombinant Human Keratin, type I cytoskeletal 19 (KRT19)	E.coli

KRT19 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP726892RA CSB-EP726892RA CSB-BP726892RA CSB-MP726892RA CSB-EP726892RA-B	Recombinant Rat Keratin, type I cytoskeletal 19 (Krt19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Code	Product Name	Source
CSB-YP716107PYX CSB-EP716107PYX CSB-BP716107PYX CSB-MP716107PYX CSB-EP716107PYX-B	Recombinant Pongo abelii Keratin, type I cytoskeletal 19 (KRT19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP012534BO CSB-EP012534BO CSB-BP012534BO CSB-MP012534BO CSB-EP012534BO-B	Recombinant Bovine Keratin, type I cytoskeletal 19 (KRT19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Potorous tridactylus (Potoroo)

Code	Product Name	Source
CSB-YP345159PRO CSB-EP345159PRO CSB-BP345159PRO CSB-MP345159PRO CSB-EP345159PRO-B	Recombinant Potorous tridactylus Keratin, type I cytoskeletal 19 (KRT19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP012534MO CSB-EP012534MO CSB-BP012534MO CSB-MP012534MO CSB-EP012534MO-B	Recombinant Mouse Keratin, type I cytoskeletal 19 (Krt19)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KRT19 ELISA Kit

KRT19 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E09606h	Human cytokeratin fragment antigen 21-1,CYFRA21-1 ELISA Kit	serum, plasma	0.078 ng/mL

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KRT19

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