MCAM

MCAM (Melanoma Cell Adhesion Molecule), also known as CD146 or MUC18, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It mediates cell-cell and cell-matrix interactions through homophilic and heterophilic binding, notably interacting with laminin-411/421 in vascular basement membranes. Functionally, MCAM activates Wnt/β-catenin and PI3K/AKT pathways to promote tumor angiogenesis, epithelial-mesenchymal transition (EMT), and metastatic progression. Overexpression of MCAM correlates with poor prognosis in melanoma, prostate cancer, and triple-negative breast cancer. It also contributes to inflammatory disorders such as multiple sclerosis by enhancing leukocyte transmigration. Therapeutic strategies targeting MCAM include monoclonal antibodies (e.g., ABX-MA1) and antibody-drug conjugates in preclinical development, with recent studies exploring its role in regulating cancer stemness and immune checkpoint interactions.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human MCAM at 2 μg/ml can bind Anti-MCAM recombinant antibody (CSB-RA013563MA1HU). The EC₅₀ is 6.107-7.906 ng/mL.

Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial(CSB-RP104394h)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

CD146 Recombinant Monoclonal Antibody(CSB-RA013563A0HU)

Validated Data

Western Blot
Positive WB detected in A375 whole cell lysate
All lanes CD146 antibody at 0.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 120 KDa

IHC image of CSB-RA013563A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-RA013563A0HU at 1:37,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Overlay histogram showing A375 cells stained with CSB-RA013563A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Human soluble cluster of differentiation 146,sCD146 ELISA Kit(CSB-E11336h)

Validated Data

Code: CSB-E11336h

Size: 96T,5×96T,10×96T

Sensitivity: 0.31 ng/mL

Detection Range: 1.25 ng/mL-80 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following MCAM reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

MCAM Antibodies

MCAM Proteins

MCAM ELISA Kits

MCAM Antibodies

MCAM Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA013563GA01HU	MCAM Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA10439C0Rb	MCAM Antibody, FITC conjugated	Human
CSB-PA10439A0Rb	MCAM Antibody	Human	ELISA, IHC
CSB-PA10439D0Rb	MCAM Antibody, Biotin conjugated	Human	ELISA
CSB-PA10439B0Rb	MCAM Antibody, HRP conjugated	Human	ELISA
CSB-PA066991	MCAM Antibody	Human	ELISA,WB,IHC
CSB-PA783399	MCAM Antibody	Human	ELISA,WB,IHC
CSB-RA013563A0HU	CD146 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF, FC

MCAM Proteins

MCAM Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP884118RA CSB-EP884118RA CSB-BP884118RA CSB-MP884118RA CSB-EP884118RA-B	Recombinant Rat Cell surface glycoprotein MUC18 (Mcam), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MCAM Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP013563HU CSB-EP013563HU CSB-BP013563HU CSB-MP013563HU CSB-EP013563HU-B	Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-CF013563HU	Recombinant Human Cell surface glycoprotein MUC18 (MCAM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-RP104394h	Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial	E.coli
CSB-MP013563HU1	Recombinant Human Cell surface glycoprotein MUC18 (MCAM), partial (Active)	Mammalian cell

MCAM Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP823168MO CSB-EP823168MO CSB-BP823168MO CSB-MP823168MO CSB-EP823168MO-B	Recombinant Mouse Cell surface glycoprotein MUC18 (Mcam), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MCAM ELISA Kit

MCAM ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E11336h	Human soluble cluster of differentiation 146,sCD146 ELISA Kit	serum, plasma, tissue homogenates	0.31 ng/mL

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