USP7

USP7, or Ubiquitin-specific peptidase 7, also known as TEF1, HAUSP, or HAFOUS, is a deubiquitinating enzyme that belongs to the peptidase C19 family, which includes ubiquitin hydrolases.
USP7 can regulate the activity of target proteins, such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), by deubiquitinating them, which counteracts the opposing ubiquitin ligase activity of proteins involved in the respective processes, such as HDM2 and TRIM27.
USP7 plays a key role in cell cycle regulation, DNA repair, chromatin remodeling, and epigenetic control. Aberrant activation or overexpression of USP7 may promote oncogenesis and viral diseases, making it a target for therapeutic intervention. USP7 is also associated with a variety of diseases, including neurodevelopmental disorders.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

USP7 Recombinant Monoclonal Antibody(CSB-RA192026A0HU)

Validated Data

Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: HAUSP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 129, 127 kDa
Observed band size: 140 kDa

IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA192026A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela Cells with CSB-RA192026A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

The following USP7 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

USP7 Antibodies

USP7 Proteins

USP7 Antibodies

USP7 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA829622	USP7 Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA849973LA01HU	USP7 Antibody	Human	ELISA, IHC, IF
CSB-PA849973LB01HU	USP7 Antibody, HRP conjugated	Human	ELISA
CSB-PA849973LC01HU	USP7 Antibody, FITC conjugated	Human
CSB-PA849973LD01HU	USP7 Antibody, Biotin conjugated	Human	ELISA
CSB-RA192026A0HU	USP7 Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF

USP7 Antibodies for Drosophila melanogaster (Fruit fly)

Code	Product Name	Species Reactivity	Application
CSB-PA894292XA01DLU	Usp7 Antibody	Drosophila melanogaster (Fruit fly)	ELISA, WB (ensure identification of antigen)

USP7 Proteins

USP7 Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP764911CH CSB-EP764911CH CSB-BP764911CH CSB-MP764911CH CSB-EP764911CH-B	Recombinant Chicken Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

USP7 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP849973HU CSB-EP849973HU CSB-BP849973HU CSB-MP849973HU CSB-EP849973HU-B	Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP849973HU	Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7),Partial	E.coli
CSB-EP849973HU1	Recombinant Human Ubiquitin carboxyl-terminal hydrolase 7 (USP7), partial	E.coli

USP7 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP700351RA CSB-EP700351RA CSB-BP700351RA CSB-MP700351RA CSB-EP700351RA-B	Recombinant Rat Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

USP7 Proteins for Drosophila melanogaster (Fruit fly)

Code	Product Name	Source
CSB-YP894292DLU CSB-EP894292DLU CSB-BP894292DLU CSB-MP894292DLU CSB-EP894292DLU-B	Recombinant Drosophila melanogaster Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

USP7 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP721329MO CSB-EP721329MO CSB-BP721329MO CSB-MP721329MO CSB-EP721329MO-B	Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 7 (Usp7), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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USP7

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