Canine brain natriuretic peptide(BNP)ELISA Kit

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Name: Canine brain natriuretic peptide(BNP)ELISA Kit
Brand: CUSABIO
SKU: CSB-E17436c

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Code: CSB-E17436c

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 12.5 pg/mL-800 pg/mL

Sensitivity: 3.12 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 34 ELISA kit; BNP-34); Brain natriuretic peptide 29 ELISA kit; BNP-29)] ELISA kit

Abbreviation

BNP

Uniprot NO.

P16859

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Type

serum, plasma, tissue homogenates

Detection Range

12.5 pg/mL-800 pg/mL

Sensitivity

3.12 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-105
1:2	Average %	91
	Range %	88-96
1:4	Average %	95
	Range %	90-102
1:8	Average %	97
	Range %	92-103

Recovery

The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-105
EDTA plasma (n=4)	96	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.867	1.953	1.910	1.706
400	1.524	1.600	1.562	1.358
200	1.166	1.124	1.145	0.941
100	0.812	0.862	0.837	0.633
50	0.524	0.563	0.544	0.340
25	0.375	0.401	0.388	0.184
12.5	0.278	0.259	0.269	0.065
0	0.203	0.204	0.204

Protocols

/uploadfile/Ins/CSB-E17436c.pdf

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Dog BNP ELISA Kit was designed for the quantitative measurement of Dog BNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.

Subcellular Location

Secreted.; [Brain natriuretic peptide 32]: Secreted.

Protein Families

Natriuretic peptide family

Tissue Specificity

Brain and also in atria, but at much lower levels than ANP.

Database Links

STRING: 9615.ENSCAFP00000024316

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