Dog Interleukin 6,IL-6 ELISA Kit

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Name: Dog Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E11260c

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Code: CSB-E11260c

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Citation （5）

Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 1.56 pg/mL-100 pg/mL

Sensitivity: 0.39 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL6

Uniprot NO.

P41323

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Type

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-100 pg/mL

Sensitivity

0.39 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	95
	Range %	89-105
1:4	Average %	100
	Range %	95-106
1:8	Average %	93
	Range %	89-97

Recovery

The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-96
EDTA plasma (n=4)	93	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.954	2.989	2.972	2.855
50	2.388	2.386	2.387	2.270
25	1.758	1.796	1.777	1.660
12.5	1.035	1.106	1.071	0.954
6.25	0.502	0.513	0.508	0.391
3.12	0.271	0.268	0.270	0.153
1.56	0.184	0.196	0.190	0.073
0	0.112	0.121	0.117

Protocols

/uploadfile/Ins/CSB-E11260c.pdf

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Dog Interleukin 6,IL-6 ELISA Kit

Description

Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in immune responses, inflammation, and hematopoiesis. Originally identified as a B-cell stimulatory factor, IL-6 regulates acute-phase responses, promotes T-cell activation, and influences the differentiation of various immune cell populations. Multiple cell types produce this cytokine, including macrophages, T-cells, endothelial cells, and fibroblasts in response to inflammatory stimuli. IL-6 signaling occurs through binding to its receptor complex, triggering downstream pathways that affect gene expression and cellular functions essential for both innate and adaptive immunity.

The Dog Interleukin 6 ELISA Kit (CSB-E11260c) is designed for quantitative measurement of canine IL-6 in serum, plasma, and tissue homogenates. This sandwich-based assay offers a detection range of 1.56-100 pg/mL with a sensitivity of 0.39 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection is performed at 450 nm wavelength, providing researchers with a reliable tool for studying IL-6 levels in canine samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in veterinary research to measure interleukin-6 levels in canine serum samples across various experimental contexts. The applications span inflammatory biomarker studies and perioperative research examining cytokine responses during medical procedures.

• Inflammatory biomarker profiling - Quantification of IL-6 alongside other acute phase proteins and pro-inflammatory cytokines including C-reactive protein, serum amyloid-A, TNF-α, and IL-1β in comprehensive inflammatory response studies
• Perioperative research - Monitoring of IL-6 concentrations in surgical contexts, including measurements before premedication, during surgery, and at multiple post-surgical timepoints to evaluate inflammatory responses to medical interventions
• Pain and analgesia studies - Analysis of cytokine levels in conjunction with endorphin measurements during tramadol administration protocols in surgical settings

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.

Gene References into Functions

An increase in ventricular dimension is accompanied by increases in AQP4 and IL-6 concentrations in the CSF of dogs with idiopathic communicating hydrocephalus. All were greatly reduced or normalised after shunt treatment. Therefore AQP4 and IL-6 in CSF can be indicators of ventriculomegaly, of cellular damage, and of improvement after the treatment of hydrocephalus. PMID:27357498
The results of this study suggest that IL-6 is a useful prognostic marker for canine critical care patients with acute internal disease. PMID:25866911
This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol. PMID:25312048
Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in dogs with syringomyelia. PMID:23659719
The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported. PMID:22381588
There was no association between IL6 variants and periodontal disease in dogs. PMID:21708229
These findings suggest that IL-6 secretion during acute urinary tract infection may serve a dual biological role by initiating the inflammatory response while also repairing urothelial defenses. PMID:21115724
Tumor-infiltrating lymphocytes isolated from regressing canine transmissible venereal tumor secrete high concentrations of IL-6 and antagonize the anti-lymphokine-activated killer activity of tumor cell transforming growth factor-beta 1. PMID:14734728
Plasma IL-6 concentration is predictive of outcome in canine systemic inflammatory response syndrome and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs. PMID:17806073
Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID:18717642