Dog Renin ELISA Kit

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Name: Dog Renin ELISA Kit
Brand: CUSABIO
SKU: CSB-E08704c

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Code: CSB-E08704c

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit

Abbreviation

REN

Uniprot NO.

Q6DYE7

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Type

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	105
	Range %	100-112
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-103
EDTA plasma (n=4)	88	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.582	2.683	2.633	2.454
2000	2.204	2.104	2.154	1.975
1000	1.451	1.521	1.486	1.307
500	0.864	0.908	0.886	0.707
250	0.569	0.584	0.577	0.398
125	0.430	0.402	0.416	0.237
62.5	0.279	0.259	0.269	0.090
0	0.182	0.176	0.179

Protocols

/uploadfile/Ins/CSB-E08704c.pdf

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Dog REN ELISA Kit was designed for the quantitative measurement of Dog REN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Gene References into Functions

With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID:19930429
Characterization of canine renin with mutations in the substrate binding site. PMID:17942478
Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID:19073902

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Subcellular Location

Secreted. Membrane.

Protein Families

Peptidase A1 family

Database Links

UNIGENE: Cfa.3701

KEGG: cfa:403838

STRING: 9615.ENSCAFP00000014146

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