Human Complement component C6(C6) ELISA kit

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Code: CSB-EL004036HU

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

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Target Background

Product Details

Alternative Names

AW111623 ELISA Kit; C6 ELISA Kit; CO6_HUMAN ELISA Kit; Complement component 6 ELISA Kit; Complement component C6 ELISA Kit

Abbreviation

Uniprot NO.

P13671

Species

Homo sapiens (Human)

Sample Type

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10000	Average %	88
	Range %	81-92
1:20000	Average %	95
	Range %	91-105
1:40000	Average %	97
	Range %	92-101
1:80000	Average %	93
	Range %	86-98

Recovery

The recovery of human C6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.021	2.107	2.064	1.940
2000	1.745	1.784	1.765	1.641
1000	1.318	1.329	1.324	1.200
500	0.902	0.894	0.898	0.774
250	0.651	0.668	0.660	0.536
125	0.489	0.492	0.491	0.367
62.5	0.337	0.341	0.339	0.215
0	0.122	0.125	0.124

Protocols

/uploadfile/Ins/CSB-EL004036HU.pdf

Troubleshooting and FAQs

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ELISA Data Analysis

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Description

This Human complement component 6 ELISA Kit was designed for the quantitative measurement of Human complement component 6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

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Target Background

Function(From Uniprot)

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells.

Gene References into Functions

Our study provided novel evidence that genetic variations in complement genes C6 and MASP1were associated with preeclampsia risk, and that the risk varied by preeclampsia subtypes PMID:27405496
recipient C6 rs9200 polymorphism is associated with HCC recurrence after OLT, and improves the predictive value of clinical models. PMID:27173880
Structure of complement C6 suggests a mechanism for initiation and unidirectional, sequential assembly of membrane attack complex (MAC). PMID:22267737
finding of very high serious morbidity in patients with complete C6 deficiency due to meningococcal infections PMID:22288589
Genetic polymorphisms in C6 gene do not influence the risk of aspirin hypersensitivity in Korean asthmatic patients. PMID:21704099
produced by trophoblast cells PMID:19665237
The most common defect, which leads to an increased susceptibility to Neisseria meningitidis infections in the Western Cape, is 879delG. PMID:17257682