Pig Angiopoietin 2,ANG-2 ELISA Kit

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Name: Pig Angiopoietin 2,ANG-2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08460p

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Code: CSB-E08460p

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 0.94 ng/mL-60 ng/mL

Sensitivity: 0.235 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit

Abbreviation

ANGPT2

Uniprot NO.

Q9BDY7

Species

Sus scrofa (Pig)

Sample Type

serum, plasma, tissue homogenates

Detection Range

0.94 ng/mL-60 ng/mL

Sensitivity

0.235 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	99
	Range %	96-102
1:4	Average %	87
	Range %	84-90
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	93	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.509	2.596	2.553	2.401
30	1.749	1.768	1.759	1.607
15	1.004	1.068	1.036	0.884
7.5	0.663	0.642	0.653	0.501
3.75	0.390	0.378	0.384	0.232
1.88	0.298	0.290	0.294	0.142
0.94	0.224	0.239	0.232	0.080
0	0.151	0.153	0.152

Protocols

/uploadfile/Ins/CSB-E08460p.pdf

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Pig ANGPT2 ELISA Kit was designed for the quantitative measurement of Pig ANGPT2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.94 ng/mL-60 ng/mL and the sensitivity is 0.235 ng/mL.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Gene References into Functions

expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID:20598355
ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID:20713984

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Subcellular Location

Secreted.

Database Links

UNIGENE: Ssc.240

KEGG: ssc:396730

STRING: 9823.ENSSSCP00000016694

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