Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Product Details

Customer Reviews and Q&A

Citations

Target Background

Home
Product
Kit
ELISA Kits
Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Name: Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit
Brand: CUSABIO
SKU: CSB-E07293r

Promotion

Code: CSB-E07293r

Rate （N/A）
Review （0）
Citation （4）

Size:

96T,5×96T,10×96T

Pls inquire

Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 62.5 pg/mL-4000 pg/mL

Sensitivity: 15.6 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>

Interested in a trial size? Please leave a message below.

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Promotion

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Alternative Names

Mif ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Glutathione-binding 13 kDa protein ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit

Abbreviation

MIF

Uniprot NO.

P30904

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	90-100
1:10	Average %	98
	Range %	92-102
1:20	Average %	94
	Range %	89-99
1:40	Average %	92
	Range %	82-96

Recovery

The recovery of rat MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	96	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.712	2.611	2.662	2.507
2000	2.054	1.953	2.004	1.849
1000	1.286	1.307	1.297	1.142
500	0.748	0.737	0.743	0.588
250	0.478	0.467	0.473	0.318
125	0.360	0.371	0.366	0.211
62.5	0.279	0.275	0.277	0.122
0	0.155	0.154	0.155

Protocols

/uploadfile/Ins/CSB-E07293r.pdf

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

■ Customer Reviews

Submit a Review here

Gender differences in oesophageal mucosal injury in a reflux oesophagitis model of rats Tomohiro Masaka et al,/,2013
The potential role of polymethyl methacrylate as a new packaging material for the implantable medical device in the bladder Kim SJ.et al,/,2015
Paralemmin-3 contributes to lipopolysaccharide-induced inflammatory response and is involved in lipopolysaccharide-Toll-like receptor-4 signaling in alveolar macrophages Chen XX.et al,/,2017
The Role of Macrophage Migration Inhibitory Factor in Remote Ischemic Conditioning Induced Hepatoprotection in A Rodent Model of Liver Transplantation Emontzpohl C, et al,/,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Subcellular Location

Secreted. Cytoplasm.

Protein Families

MIF family

Tissue Specificity

Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.

Database Links

UNIGENE: Rn.2661

KEGG: rno:103694877

STRING: 10116.ENSRNOP00000008608

Promotions