Rat P-Selectin ELISA kit

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Name: Rat P-Selectin ELISA kit
Brand: CUSABIO
SKU: CSB-E07399r

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Code: CSB-E07399r

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Citation （15）

Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 9.38 ng/mL-600 ng/mL

Sensitivity: 2.34 ng/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P

Abbreviation

SELP

Uniprot NO.

P98106

Species

Rattus norvegicus (Rat)

Sample Type

serum, plasma, tissue homogenates

Detection Range

9.38 ng/mL-600 ng/mL

Sensitivity

2.34 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	92
	Range %	84-105
1:4	Average %	97
	Range %	92-103
1:8	Average %	94
	Range %	82-98

Recovery

The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	95	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
600	2.755	2.812	2.784	2.616
300	2.217	2.236	2.227	2.059
150	1.545	1.524	1.535	1.367
75	1.051	0.979	1.015	0.847
37.5	0.587	0.622	0.605	0.437
18.75	0.456	0.438	0.447	0.279
9.38	0.288	0.279	0.284	0.116
0	0.173	0.162	0.168

Protocols

/uploadfile/Ins/CSB-E07399r.pdf

Troubleshooting and FAQs

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Rat P-Selectin ELISA kit

Description

The Rat P-Selectin ELISA kit is a powerful tool for researchers in the field of cardiovascular research. This ELISA kit allows for the quantitative measurement of P-selectin levels in a range of sample types from Rattus norvegicus (Rat), including serum, plasma, and tissue homogenates.

With a detection range of 9.38 ng/mL to 600 ng/mL and a sensitivity of 2.34 ng/mL, this ELISA kit delivers accurate and precise results, making it an essential tool for inflammation, immunology and cardiovascular research. The assay time is only 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

The Rat P-Selectin ELISA kit utilizes a sandwich assay principle, where the P-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

This ELISA kit has been cited in over 10 publications, highlighting its reliability and utility in inflammation, immunology and cardiovascular research. It is a valuable addition to any research laboratory studying inflammation, immunology and cardiovascular research, providing accurate and reliable measurement of P-selectin levels in rat samples.

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■ Q&A

Hello,
please let me know whether this elisa kit can be use to detect the P selectin which is released from platelet or not.

Thanks for your inquiry.
Sorry we did not ever validate it before.
We suggest you do pre-testing first and determine it by yourself.

The application of tetrahydroisoquinoline-3-carbonyl-TARGD (F) F as an anti-thrombotic agent having dual mechanisms of action Yang G et al,/,2012
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,/,2015
Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis Wang Y. et al,/,2015
IQCA-TASS: a nano-scaled P-selectin inhibitor capable of targeting thrombus and releasing IQCA/TARGD (S) S in vivo Jianhui Wu.et al,/,2016
Protective role of B-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation Jia-Zhen Wu.et al,/,2017
IQCA-TAVV: To explore the effect of P-selectin, GPIIb/IIIa, IL-2, IL-6 and IL-8 on deep venous thrombosis Wu J.et al,/,2017
ATIQCTPC: a nanomedicine capable of targeting tumor and blocking thrombosis in vivo Xu X.et al,/,2017
Shape memory and hemostatic silk-laponite scaffold for alveolar bone regeneration after tooth extraction trauma H Zhou,/,2024
Assessment of Thrombotic Risk in Atrial Fibrillation with Ultrasound Molecular Imaging of P-Selectin Jing Y.et al,/,2018
Heptapeptide-based modification leading to enhancing the action of MTCA on activated platelets, P-selectin, GPIIb/IIIa Ze Li.et al,/,2018
DM-induced Hypermethylation of IR and IGF1R attenuates mast cell activation and airway responsiveness in rats D Fu,/,2020
Docking of THPDTPI: to explore P-selectin as a common target of anti-tumor, anti-thrombotic and anti-inflammatory agent Zhu H,/,2023
Metformin regulates the effects of IR and IGF-1R methylation on mast cell activation and airway reactivity in diabetic rats with asthma through miR-152-3p/DNMT1 axis D Fu,/,2023
Regulation of the Hemostatic Performance of Collagen: On the Role of Hydrophilicity J Zhang, L Qiu, H Xu, Y Liu, B Wei, H Kou, C Xu,/,/
Impact of gardenia extract on coagulation function in rats with acute myocardial ischemia model Z Zhu, S Mo, J Luo, Q Yang,/,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.

Gene References into Functions

Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID:22575043
Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID:22340239
Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID:21180132
Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID:19780886
Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID:18471420
P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID:12081568
P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID:12100021
Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID:12201360
P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID:12377736
Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID:14963004
P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID:15742404
expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID:15888973
P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID:17488661

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Selectin/LECAM family

Tissue Specificity

Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.

Database Links

UNIGENE: Rn.10012

KEGG: rno:25651

STRING: 10116.ENSRNOP00000003759

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