Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

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Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Name: Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
Brand: CUSABIO
SKU: CSB-E13161Sh

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Code: CSB-E13161Sh

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Size:

96T,5×96T,10×96T

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Quantity:

Sample Types: serum, plasma, tissue homogenates

Detect Range: 5 μIU/mL-2000 μIU/mL

Sensitivity: 5 μIU/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit

Abbreviation

PRL

Uniprot NO.

P01240

Species

Ovis aries (Sheep)

Sample Type

serum, plasma, tissue homogenates

Detection Range

5 μIU/mL-2000 μIU/mL

Sensitivity

5 μIU/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Competitive

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	94
	Range %	84-101
1:4	Average %	93
	Range %	85-103
1:8	Average %	90
	Range %	87-101

Recovery

The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-104
EDTA plasma (n=4)	95	88-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














μIU/ml	OD1	OD2	Average
2000	0.154	0.149	0.152
375	0.338	0.351	0.345
62.5	0.542	0.572	0.557
17.5	0.983	0.959	0.971
5	1.515	1.529	1.522
0	2.164	2.077	2.121

Protocols

/uploadfile/Ins/CSB-E13161Sh.pdf

Troubleshooting and FAQs

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ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

This Sheep PRL ELISA Kit was designed for the quantitative measurement of Sheep PRL protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 μIU/mL-2000 μIU/mL and the sensitivity is 5 μIU/mL.

■ Customer Reviews

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Prepartum and Postpartum Feed Restrictions Affect Blood Metabolites and Hormones Reducing Colostrum and Milk Yields in Fat-Tailed Dairy Sheep M Zarri,/,2021

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation.

Gene References into Functions

These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway. PMID:27836773
Prolactin induces apoptosis of lactotropes PMID:24859278
Prolactin and epidermal growth factor stimulate adipophilin synthesis in HC11 mouse mammary epithelial cells via the PI3-kinase/Akt/mTOR pathway. PMID:22426621
Differences in accessibility of the rat arcuate nucleus to sheep prolactin, together with intrinsic differences in neuroendocrine dopaminergic neurons, facilitate homeostatic feedback regulation of sheep prolactin release in the rat hypothalamus PMID:21953590
conclude that PRL is an important part of the regulation of the pituitary-gonadal system in moderately seasonal DLS rams; it seems to impair release of LH from gonadotrophs, help maintain functional integrity of Leydig cells, & promote testicular growth PMID:19755483
increased PRL concentration within the central nervous system (CNS) for a few days before oncoming ovulation has no inhibitory effect on tonic LH secretion PMID:15127327
findings provide strong evidence that the circannual regulation of prolactin secretion, and its associated biology, derive from a pituitary-based timing mechanism PMID:17185605
Prolactin expressed in adult and fetal sheep brain could be involved in neurodevelopment and/or modulation of the neuroendocrine stress axis. PMID:18223310
These results demonstrate that TGF-beta abrogates prolactin-stimulated beta-casein gene expression in mammary epithelial cells through, at least in part, a Smad3-dependent mechanism. PMID:18335503
circulating prolactin decreased (P<0.001) in response to leptin during long days. PMID:18451065
DNA polymorphism of 5' flanking region of prolactin gene and its association with litter size in sheep. PMID:19207932
PRL exerts protective effects on adult neurogenesis in the dentate gyrus under chronic stress conditions in mice. PMID:19211889