Abbreviation
Recombinant Human GDF6 protein (Active)
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Less than 0.1 EU/ug as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 2.0 μg/ml, corresponding to a specific activity of > 500 IU/mg.
Research Area
Signal Transduction
Alternative Names
GDF6; BMP13; GDF16Growth/differentiation factor 6; GDF-6; Bone morphogenetic protein 13; BMP-13; Growth/differentiation factor 16
Species
Homo sapiens (Human)
Expression Region
336-455aa
Target Protein Sequence
TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
GDF6 (BMP-13) plays a critical role in joint, tendon, and cartilage development, making it a key tool for investigating chondrogenic and osteogenic differentiation pathways. This tag-free recombinant human GDF6, encompassing the full mature protein (aa 336–455), demonstrates robust biological activity with an ED50 below 2.0 μg/ml in ATDC5 alkaline phosphatase induction assays, corresponding to a specific activity exceeding 500 IU/mg—data that supports use in stem cell differentiation studies, receptor-ligand binding assays via SPR or ELISA, and in vivo cartilage regeneration models. The E. coli expression system produces a non-glycosylated homodimer that enables precise structure-activity analyses without glycan heterogeneity, while purity greater than 95% by SDS-PAGE combined with endotoxin levels below 0.1 EU/μg satisfies the criteria typical for cell-based differentiation assays and sensitive in vivo applications.
