Abbreviation
Recombinant Human IL4 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0x106 IU/mg.
Alternative Names
B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; B-cell stimulatory factor 1; BCGF 1; BCGF1; Binetrakin; BSF-1; BSF1 ; IGG1 induction factor; IL 4; IL-4; IL4; IL4_HUMAN; Il4e12; Interleukin 4; Interleukin 4 variant 2; Interleukin 4; isoform 1 ; Interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra
Species
Homo sapiens (Human)
Expression Region
25-153aa
Target Protein Sequence
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-4 is a pleiotropic cytokine central to Th2 polarization, B-cell class switching, and alternative macrophage activation, making signaling potency and endotoxin control essential for meaningful experimental results. This tag-free recombinant human IL-4, encompassing the full mature sequence (residues 25–153), demonstrates an ED50 below 0.2 ng/ml in TF-1 cell proliferation assays—corresponding to a specific activity exceeding 5.0 × 10⁶ IU/mg—which supports use in JAK1/STAT6 signaling pathway studies, Th2 differentiation assays, and primary B-cell activation experiments where precise dose-response relationships are critical. Endotoxin levels below 1.0 EU/μg, confirmed by LAL testing, minimize LPS-driven artifacts that can confound monocyte and dendritic cell functional readouts, making this preparation appropriate for in vivo inflammation models and as a reference standard in cytokine detection assays. Purity exceeding 98% by SDS-PAGE further provides a suitable basis for antibody validation workflows, including use as a coating antigen in ELISA or a positive control in western blotting.
