Abbreviation
Recombinant Mouse Il5 protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of >5.0x105 IU/mg.
Alternative Names
Il5; Il-5Interleukin-5; IL-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF
Species
Mus musculus (Mouse)
Expression Region
21-133aa
Target Protein Sequence
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 m filtered 20 mM Tris, pH 9.0, 150 mM NaCl
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interleukin-5 is a central regulator of eosinophil differentiation, activation, and survival, making it a critical target in studies of allergic inflammation and type 2 immune responses. This tag-free recombinant mouse IL-5, encompassing the full mature sequence (residues 21–133), demonstrates strong signaling potency with an ED50 below 2 ng/ml in TF-1 cell proliferation assays, corresponding to a specific activity exceeding 5.0 × 10⁵ IU/mg — a level that supports use in JAK/STAT signaling pathway dissection, eosinophil differentiation studies, and as a reference standard in cytokine detection assays. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 98% by SDS-PAGE provides a suitable basis for in vivo murine models of airway inflammation and for antibody development workflows including immunization, ELISA coating, and positive control applications.
