Abbreviation
Recombinant Rhesus macaque IL1B protein (Active)
Purity
>98% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10.G4.1 cells is less than 10 pg/ml, corresponding to a specific activity of >1.0x108 IU/mg.
Alternative Names
IL1BInterleukin-1 beta; IL-1 beta
Species
Macaca mulatta (Rhesus macaque)
Expression Region
117-269aa
Target Protein Sequence
APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
IL-1β is a master regulator of NF-κB and MAPK signaling cascades that drive inflammation, making species-matched reagents essential for translational primate immunology studies. This tag-free recombinant rhesus macaque IL-1β, expressed in *E. coli* as the full-length mature protein (117–269 aa), demonstrates exceptional signaling potency with an ED50 below 10 pg/mL in D10.G4.1 proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁸ IU/mg. Endotoxin levels below 1.0 EU/μg ensure that downstream immune cell responses—including NF-κB pathway activation, T-cell proliferation, and macrophage differentiation assays—reflect genuine IL-1β signaling rather than LPS-driven artifacts. With purity above 98% by SDS-PAGE and stringent endotoxin control, this protein provides a suitable basis for use as a reference standard in cytokine detection assays, a functional immunogen for antibody development, and a reliable tool in nonhuman primate *in vivo* inflammation models.
