Abbreviation
Recombinant Human NECTIN4 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Anti-Human Nectin-4 mAb-mFc at 2μg/ml can bind Human Nectin-4-His, the ED50 of Human Nectin-4-His is 16.29 ng/ml.
Research Area
Cell Biology
Alternative Names
DKFZp686K05193; EDSS1; Ig superfamily receptor LNIR; Nectin 4; Nectin-4; poliovirus receptor-related 4; Processed poliovirus receptor-related protein 4; PRR4 ; pvrl4; PVRL4_HUMAN
Species
Homo sapiens (Human)
Expression Region
32-351aa
Target Protein Sequence
GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20mM Tris-HCl, 8% Sucrose, 0.05% Tween 80, pH 8.0.
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Description
Nectin-4 plays a central role in cell-cell adhesion and has emerged as a key therapeutic target in tumor metastasis research, making functionally validated protein reagents essential for mechanistic studies. This recombinant human Nectin-4 construct spans residues 32–351, covering the extracellular immunoglobulin-like domains critical for trans-interactions with binding partners such as Nectin-1. Produced in mammalian cells to promote native-like glycosylation and proper folding, the protein demonstrates robust binding activity with an ED50 of 16.29 ng/ml in a functional ELISA against an anti-human Nectin-4 mAb-mFc, supporting use in antibody development and validation, cell adhesion and spreading assays, and cell migration and invasion studies relevant to immune cell trafficking. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typically required for sensitive cell-based assays investigating tumor metastasis mechanisms and integrin-ligand binding interactions.
