Abbreviation
Recombinant Mouse Dsg3 protein, partial (Active)
Purity
Greater than 85% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5 μg/mL can bind Anti-DSG3 recombinant antibody (CSB-RA007205A0HU), the EC50 is 2.624-3.182 μg/mL.
Research Area
Signal Transduction
Alternative Names
(Desmoglein-3)(130 kDa pemphigus vulgaris antigen homolog)
Molecular Characterization
Species
Mus musculus (Mouse)
Expression Region
50-617aa
Target Protein Sequence
EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Dsg3 at 5 μg/ml can bind Anti-DSG3 recombinant antibody (CSB-RA007205A0HU), the EC50 is 2.624-3.182 μg/ml.
Description
Desmoglein-3 mediates calcium-dependent cell-cell adhesion in stratified epithelia and serves as the primary autoantigen in pemphigus vulgaris, making it a critical target for antibody development and desmosomal signaling research. This recombinant mouse Dsg3 construct spans residues 50–617, encompassing the extracellular cadherin domains essential for homophilic and heterophilic binding interactions, and its mammalian expression system supports native-like glycosylation and folding required for functional studies. Binding activity has been validated by ELISA, where immobilized Dsg3 at 5 μg/mL bound an anti-DSG3 recombinant antibody with an EC50 of 2.624–3.182 μg/mL, confirming correct epitope presentation suitable for antibody validation, cell adhesion and spreading assays, and integrin-ligand binding studies. With purity exceeding 85% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the quality thresholds commonly required for cell-based migration and invasion assays investigating desmosomal disruption in autoimmune or metastasis contexts.
