| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IHC | 1:20-1:200 |
| IF | 1:50-1:200 |
Western Blot
All lanes: CALHM3 antibody at 8µg/ml + Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40, 39 kDa
Observed band size: 40, 55 kDa
Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA768231LA01HU at dilution of 1:100
Immunofluorescent analysis of U251 cells using CSB-PA768231LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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