DGKG Antibody

Code: CSB-PA006836LA01HU

Rate （N/A）
Review （0）

Size:

20μg50μg100μg

US$83

Quantity:

Species Reactivity: Human, Mouse

Raised in: Rabbit

Application: ELISA, WB

Send an Inquiry

Start an on-line Chat

Online ordering is currently available for U.S. customers only. For orders outside the U.S., please kindly submit an inquiry or start a chat with us.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P49619

Target Names

DGKG

Alternative Names

DGKG antibody; DAGK3Diacylglycerol kinase gamma antibody; DAG kinase gamma antibody; EC 2.7.1.107 antibody; Diglyceride kinase gamma antibody; DGK-gamma antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Diacylglycerol kinase gamma protein (1-255AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: DGKG antibody at 4µg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 90, 87, 85 kDa
Observed band size: 90 kDa

Description

To produce the polyclonal antibody that targets DGKG, rabbits were immunized with the recombinant human DGKG protein (1-255aa). Afterward, this DGKG polyclonal antibody was purified using protein G affinity chromatography, resulting in a purity level exceeding 95%. It can recognize and bind to DGKG protein present in human and mouse samples.

This DGKG polyclonal antibody has undergone rigorous validation and is appropriate for use in ELISA and WB. As such, it can be utilized to assess DGKG protein levels and detect its presence and size in different experimental contexts.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has no apparent specificity with regard to the acyl compositions of diacylglycerol. Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination.

Gene References into Functions

Epigenetic silencing of DGKG expression is associated with skin cancer. PMID:28218473
these results indicate that DGKG is an anionic phospholipid binding protein that preferably interacts with a small highly charged head group that is very close to the glycerol or sphingosine backbone. PMID:24486543
diacylglycerol kinase gamma regulates macrophage differentiation. PMID:12732202
the interaction of diacylglycerol kinase gamma with the Src homology 2 and C1 domains of beta2-chimaerin is induced synergistically by Phorbol ester and hydrogen peroxide PMID:17803461