| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:20-1:200 |
| IF | 1:50-1:200 |
Western blot
All lanes: IFI44 antibody at 6µg/ml + Mouse lung tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 51, 15 kDa
Observed band size: 51 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA011014LA01HU at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using CSB-PA011014LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
The IFI44 polyclonal antibody was prepared by inoculating a rabbit with recombinant human 5-hydroxytryptamine receptor 1E protein (203-291aa). After extracting antibodies from the rabbit serum, protein G affinity chromatography purification was performed to obtain a highly pure antibody with over 95% purity and minimal impurities.
This IFI44 polyclonal antibody is capable of effectively recognizing and binding to IFI44 protein from both human and mouse samples. Validation has been conducted for this antibody's use in various experimental settings, including ELISA, WB, IHC, and IF. Researchers can employ this antibody to detect IFI44 protein levels, identify its presence and size, and analyze its distribution.
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