TNF Antibody

Code: CSB-PA023955DA01SH

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US$83

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Species Reactivity: Sheep

Raised in: Rabbit

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P23383

Target Names

TNF

Alternative Names

TNF antibody; TNFA antibody; TNFSF2 antibody; Tumor necrosis factor antibody; Cachectin antibody; TNF-alpha antibody; Tumor necrosis factor ligand superfamily member 2 antibody; TNF-a) [Cleaved into: Tumor necrosis factor antibody; membrane form antibody; N-terminal fragment antibody; NTF); Intracellular domain 1 antibody; ICD1); Intracellular domain 2 antibody; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor antibody; soluble form] antibody

Raised in

Rabbit

Species Reactivity

Sheep

Immunogen

Recombinant Sheep Tumor necrosis factor protein (78-234AA)

Immunogen Species

Ovis aries (Sheep)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot
All lanes: Tumor necrosis factor antibody at dilution of 1: 1000
Lane 1: Tumor necrosis factor protein at 0.05μg
Lane 2: Tumor necrosis factor protein at 0.5μg
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa

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Target Background

Function(From Uniprot)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Gene References into Functions

The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID:25817420
The p38 MAPK inhibitor decreased (P < 0.01) gene expression of TNFa but its effect was not observed at the level of TNFa protein synthesis PMID:24995301
TNF-alpha gene expression in multifidus was greater on both sides in the disc lesion animals than in the muscle of control animals. PMID:24718080
Injection of peptidoglycan-polysaccharide resulted in fever, increased concentrations of TNF-alpha on the day of and the day after challenge, and decreased concentrations of progesterone on days 14 and 21. PMID:20603027
These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID:15899940
plasma IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor prognosis of pregnancy toxaemia in ewes PMID:17221363

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.

Protein Families

Tumor necrosis factor family

Database Links

UNIGENE: Oar.455

KEGG: oas:443540

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