WDR73 Antibody

Code: CSB-PA744047LA01HU

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20μl50μl100μl

US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Target Background

Product Details

Uniprot NO.

Q6P4I2

Target Names

WDR73

Alternative Names

WDR73 antibody; HSPC264WD repeat-containing protein 73 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human WD repeat-containing protein 73 protein (163-366AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-4 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate
All lanes: WDR73 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
IHC image of CSB-PA744047LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of SH-SY5Y cells with CSB-PA744047LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Description

The antigen affinity purified Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody is generated in rabbits using Recombinant Human WD repeat-containing protein 73 (163-366aa) as the immunogen. This product is tested for use in WB, ELISA, IHC, and IF.
The target, WDR73, belongs to one of the largest protein families, the WD40-repeat proteins. It may play a significant role in the functions of microtubule and spindle poles during mitotic cell division. Aberrations in its actions are linked to Galloway-Mowat Syndrome. Also, the reduced expression of the WDR73 gene has been linked to irregular nuclear structure and brain structure aberrations.
The Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody reacts with Human WDR73. Thus, it is used to study the role of WDR73 in Galloway-Mowat Syndrome and some other brain defects.

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Target Background

Function(From Uniprot)

May play a role in the regulation of microtubule organization and dynamics.

Gene References into Functions

We expanded the clinical phenotype of GMS with WDR73 gene defect to include retinal dysfunction with missense mutation and developmental dysplasia of the hip. PMID:29929488
WDR73 as a candidate gene of severe intellectual disability and cerebellar hypoplasia. PMID:27983999
We document postnatal onset of CA, a retinopathy, basal ganglia degeneration, and short stature as novel features of WDR73-related disease, and define WDR73-related disease as a new entity of infantile neurodegeneration. PMID:26123727
Nonsense mutation in the WDR73 gene is associated with Galloway-Mowat syndrome PMID:25873735
WDR73 interacts with mitotic microtubules to regulate cell cycle progression, proliferation and survival in brain PMID:26070982
WDR73 plays a crucial role in the maintenance of cell architecture and cell survival. PMID:25466283

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Subcellular Location

Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow.

Protein Families

WD repeat WDR73 family

Tissue Specificity

Expressed in kidney and brain. In the kidney, expressed in glomeruli, most probably in podocytes, and in tubules (at protein level). In the brain, expressed in the cerebellum, with high levels in Purkinje cells and their projecting axons, in the deep cere

Database Links

HGNC: 25928

UNIGENE: Hs.745027

KEGG: hsa:84942

STRING: 9606.ENSP00000387982

OMIM: 251300

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