Recombinant Mouse Angiomotin (Amot), partial

Code
MSDS	MSDS Datasheet
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Source
Conjugate
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Target Background

Product Details

Abbreviation

Amot

Purity

>85% (SDS-PAGE)

Target Names

Amot

Uniprot NO.

Q8VHG2

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1-1126

Target Protein Sequence

MRSSDDQPSG GTTVLQRLLQ EQLRYGNPSE NRSLLAIHQQ ATGNSSPFST GSGNQGPQND VLSSQDHHQQ QLVAHPARQE PQGQEIQSEN GVMEKQLSPR MQNNEELPTY EEAKVQSQYF RGQQHASVGA AFYVTGVTNQ KMRTEGRPSV QRLTPGKMHQ DEGLRDLKQG HVRSLSERLM QMSLATSGVK AHPPVTSAPL SPPQPNDLYK NATSSSEFYK AQGPPPSQHS LKGMEHRGPP PEYPFKGVPS QSVVCKSQEP GHFYSEHRLN QPGRTEGQLM RYQHPPEYGA ARATQDISSL SLSARNSQPH SPTSSLTAGA SSLPLLQSPP STRLPPGQHL VSNQGDHSAH LSRHQQHLLS SQSHQGDHYR HAQASLTSAQ QQPGEAYSAM PRAQQSASYQ PMPADPFAMV SRAQQMVEIL SDENRNLRQE LDGCYEKVAR LQKVETEIQR VSEAYENLVK SSSKREALEK AMRNKLEGEI RRMHDFNRDL RDRLETANKQ LAEKEYEGSE DTRKTISQLF AKHKENQREK EKLEAELATA RSTNEDQRRH IEIRDQALSN AQAKVVKLEE ELKKKQVYVD KVEKMQQALV QLQAACEKRE QLEHRLRTRL ERELESLRIQ QRQGNSQPTN ASEYNAAALM ELLREKEERI LALEADMTKW EQKYLEENVM RHFALDAAAT VAAQRDTTVI SHSPNTSYDT ALEARIQKEE EEILMANKRC LDMEGRIKTL HAQIIEKDAM IKVLQQRSRK EPSKTEQLSS MRPAKSLMSI SNAGSGLLAH SSTLTGAPIM EEKRDDKSWK GSLGVLLGGD YRVEPVPSTP SPVPPSTPLL SAHSKTGSRD CSTQTERGPE STKTAAVTPI SAPMAGPVAA AAPAAAINAT AATNTATAAT NTTIMVAAAP VAVAAVAAPA AAAATPSPAN AAALAAAAAP ATSVSAATSV SAANSISPAA PVAPAAVVPP AAPVSPAAAV QIPAAASLTP ATVSPTAATA TAAVAAATTA AITAAAAAAT TAIQVAPATS APVPSPASIP APATAQASAP TPTQASTPAP TEPPSPVPTP TPALVQTEGP ANPGASSGPR RLSTPNLMCN PDKPDAPAFH SSTLERKTPI QILGQEPDAE MVEYLI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.

Gene References into Functions

Rho attenuates the interaction between Amot and Nf2 by binding to the coiled-coil domain of Amot. PMID:28947533
a new function of RNF146 and tankyrase in stabilizing the Crumbs complex through downregulation of AMOT proteins at the apical membrane, is reported. PMID:27521426
AMOT is a crucial suppressor of lung cancer metastasis and highlight its critical role as a tumor suppressor and its potential as a prognostic biomarker and therapeutic target for lung cancer. PMID:25381822
The loss of Angiomotin, together with Angiomotin-like 2, leads to differentiation of inner cell mass cells and compromised peri-implantation development. PMID:23903990
The phosphorylation of S176 in the N-terminal domain of Amot is a critical step for activation of the Hippo pathway in adherens junctions and cell polarity disconnects the Hippo pathway from cell-cell adhesion by sequestering Amot from AJs. PMID:23791731
Amot, Amotl1, and Amotl2 are differentially expressed in uterine cells during the peri-implantation period. PMID:22813598
A vaccine targeting angiomotin induces an antibody response which alters tumor vessel permeability and hampers the growth of established tumors. PMID:22426512
Depletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo PMID:21481793
Angiomotin may promote neoplastic angiogenesis by both stimulating invasion as well as stabilizing established tubes. PMID:14730344
angiomotin, in addition to controlling cell motility, may play a role in the assembly of endothelial cell-cell junctions PMID:16043488
p80- and p130-angiomotin play coordinating roles in vascular tube formation by affecting cell migration and cell shape, respectively. PMID:16640563
Data show that MUPP1 interacts with angiomotin (Amot), JEAP/Amot-like 1 and MASCOT/Amot-like 2, and that all the Amot/JEAP family proteins also interacted with Patj, a close relative of MUPP1. PMID:17397395
75% of Amot knockout mice die between embryonic day 11 (E11) and E11.5 and exhibit severe vascular insufficiency in the intersomitic region as well as dilated vessels in the brain. PMID:17699752
Homo-oligomerization of p80-Amot and hetero-oligomerization of both isoforms are critical for a switch between a migratory and a non-migratory cell phenotype in endothelial cells. PMID:18164266
Amot and AmotL1 have similar effects on endothelial migration and tight junction formation in vitro. In vivo Amot appears to control the cell polarity and AmotL1 affects the stability of cell-cell junctions. PMID:19590046