Recombinant Mouse Vinculin (Vcl), partial

1. Vinculin is necessary to maintain the integrity of the glomerular filtration barrier by modulating podocyte foot processes and stabilizing intercellular junctions. PMID:29241625
2. Vinculin-M94I resulted in 37% reduction in peak sodium current compared to wild type and the combination of Vinculin-M94I and pH 7.0 decreased peak sodium current. PMID:28218286
3. Lipid binding increases vinculin activation and turnover at focal adhesions. PMID:28089450
4. results suggest that the vinculin-actin interaction participates in FA-mediated mechanotransduction PMID:28388663
5. These results suggest that vinculin promotes the nuclear localization of transcription factor TAZ to inhibit the adipocyte differentiation on rigid extracellular matrix. PMID:28115535
6. These data demonstrate that Src-mediated phosphorylation is necessary for vinculin activation, and that phosphorylation controls cytoskeletal mechanics by regulating force transmission between the actin cytoskeleton and focal adhesion proteins. PMID:26240176
7. results suggest that vinculin promotes directionally persistent cell migration and tension-dependent ECM remodeling in complex 3D environments by increasing cell-ECM adhesion and traction force generation. PMID:26195589
8. Force-dependent conformational switch of alpha-catenin controls vinculin binding. PMID:25077739
9. Mutant vinculin expressing cells are altered in cell migration, which is accompanied by changes in cell adhesion. PMID:25358683
10. show that Src phosphorylation of Y1065 within the C-terminal hairpin regulates Vt-mediated actin bundling and provide a detailed characterization of vinculin Y1065 mutations PMID:25115937
11. The E29R mutation might prime the vinculin head for F-actin binding, which results in higher cell stiffness, contractile force, and strengthening of focal adhesions. PMID:25193699
12. serine phosphorylation is required for the activation of vinculin and force transmission in focal adhesions. PMID:24996180
13. VCL binding talin, but not Arp2/3, is critical for osteoclast function, and its selective inhibition retards physiological bone loss. PMID:24675074
14. Crk-associated substrate -vinculin binding significantly affects the dynamics of Crk-associated substrate and vinculin within focal adhesions as well as the size of focal adhesions. PMID:23974298
15. This study demonistrated that Deletion of the vinculin disturbs the localization of GFAP in Bergmann glial cells in mice. PMID:23595642
16. These results identify vinculin as a major controller of integrin-mediated adhesion dynamics in B cells PMID:23872053
17. role for vinculin in ventricular myocyte mechanics and dysfunction PMID:23561539
18. Vinculin organizes lamellipodia and lamella F-actin and mediates F-actin retrograde flow engagement at focal adhesion to generate extracellular matrix traction. PMID:23836933
19. Data indicate that vinculin is not required for transmission of adhesive and traction forces but is necessary for myosin contractility-dependent adhesion strength and traction force and for the coupling of cell area and traction force. PMID:23716647
20. vinculin controls the transmission of intracellular and extracellular mechanical cues that are important for the spatiotemporal assembly, disassembly, and reorganization of focal adhesions to coordinate polarized cell motility PMID:23375895
21. alphaE-catenin recruits vinculin to adherens junctions more effectively than alphaN-catenin, partly because of its higher affinity for actin filaments. PMID:23589308
22. alpha-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength PMID:23266828
23. Results indicate a tissue-specific function of vinculin in cartilage whereby it controls chondrocytic differentiation. PMID:22416133
24. sca4 activates vinculin and interacts with the actin cytoskeleton; vinculin has roles in Rickettsia pathogenesis PMID:21841197
25. These results demonstrate that vinculin's mechano-coupling function is dependent on its conformational state. PMID:21295550
26. vinculin is not required for the traditional functions of alpha(IIb) beta(3) or the platelet actin cytoskeleton PMID:20670372
27. Data show that mutants of vinculin that are unable to interact with PtdIns(4,5)P, attenuate PIP5K- and LPA-induced neurite retraction. PMID:20841379
28. vinculin is an indispensable factor determining HSC repopulation capacity, independent of integrin functions. PMID:20663867
29. Vinculin-DeltaEx20 is an active variant supporting adhesion site stabilization without an enhanced mechanical coupling. Its presence in a transgenic animal reveals the potential of splice variants in the vinculin gene to alter vinculin function in vivo. PMID:20644727
30. phosphorylation on position Y1065 is essential for accurate incorporation of vinculin into FAs and mechanical behavior of cells. PMID:20678470
31. Vinculin facilitates cell invasion into three-dimensional collagen matrices. PMID:20181946
32. These results indicate that contractility modulates adhesion strengthening via FAK-dependent, vinculin-containing focal adhesion assembly. PMID:20205236
33. The phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. PMID:20308429
34. Both the lipid binding and the src-phosphorylation of vinculin's C-terminus are important for cell mechanical behavior. PMID:20006947
35. Vinculin found required for control of cell shape, cell mechanics, and rac-dependent lamellipodia formation PMID:11785963
36. Integrin-mediated adhesion does not require vinculin to couple with focal adhesion proteins for mechano-chemical signaling in embryonic F9 carcinoma cells. PMID:11991656
37. Vinculin regulates survival and motility via ERK by controlling the accessibility of paxillin for FAK interaction. PMID:15138291
38. model in which unfurling of the metavinculin tail domain is impaired by the negative charges of the 68-amino acid insert, thus requiring vinculin to fully activate the metavinculin molecule PMID:15159399
39. Wild-type vinculin and single tyrosine phosphorylation mutant proteins Y100F and Y1065F were significantly more effective at rescuing the spreading defect of vinculin null cells than the double mutant Y100F/Y1065F. PMID:15229287
40. Heterozygous inactivation of the vinculin gene predisposes to stress-induced cardiomyopathy PMID:15331426
41. studies indicate that PTEN protein levels are dependent on the maintenance of beta-catenin-MAGI-2 interaction, in which vinculin plays a critical role PMID:15579911
42. The Vinculin localized at costameres and intercalated discs. PMID:15679099
43. activated vinculin localized at focal adhesions recruits vinexins to focal adhesions PMID:16126177
44. The results suggest that vinculin inhibits cell migration by stabilising FAs, and that binding of inositol phospholipids to Vt plays an important role in FA turnover. PMID:16584805
45. This is the first report of tissue-specific inactivation of the Vcl gene and shows that it is required for preservation of normal cell-cell and cell-matrix adhesive structures. PMID:17785437
46. data demonstrate that vinculin contributes to the mechanical stability under large external forces by regulating contractile stress generation. PMID:17890382
47. The expression of vinculin constructs with unmasked binding sites in the head and tail regions induces dramatic focal adhesion growth, which is mediated by their direct interaction with talin. PMID:18056416
48. Abl differentially regulates Crk-associated substrate, vinculin, and paxillin in arterial vessels PMID:19542491

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UNIGENE: Mm.279361

KEGG: mmu:22330

STRING: 10090.ENSMUSP00000022369