KLKB1 Recombinant Monoclonal Antibody

Code: CSB-RA553053A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P03952

Target Names

KLKB1

Alternative Names

Plasma kallikrein (EC 3.4.21.34) (Fletcher factor) (Kininogenin) (Plasma prekallikrein) (PKK) [Cleaved into: Plasma kallikrein heavy chain, Plasma kallikrein light chain], KLKB1, KLK3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human KLKB1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA553053A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Description

Plasma kallikrein, encoded by the KLKB1 gene, serves as a critical serine protease within the contact activation system, where it participates in the intrinsic coagulation cascade and the kallikrein-kinin pathway. This enzyme plays essential roles in blood coagulation, inflammation, and blood pressure regulation through its generation of bradykinin from kininogen substrates. Dysregulation of plasma kallikrein activity has been implicated in cardiovascular disease, hereditary angioedema, and various malignancies, making it a compelling target for researchers investigating thrombosis, inflammatory signaling, and tumor microenvironment dynamics.

This recombinant monoclonal antibody, clone 9F6, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived reagents. The antibody was raised against a synthetic peptide derived from human KLKB1 and has been affinity-purified to ensure high specificity for your target.

Validation studies confirm reliable performance in ELISA and immunohistochemistry applications. IHC testing in paraffin-embedded human liver tissue using a citrate buffer antigen retrieval method and HRP-polymer detection demonstrated clear target visualization at a 1:100 dilution, with recommended working concentrations ranging from 1:50 to 1:200 depending on tissue type and detection system sensitivity.

Whether your research focuses on cardiovascular biology, cancer progression, or signal transduction mechanisms involving the contact activation pathway, this antibody provides a dependable tool for detecting KLKB1 in human samples with the precision that meaningful experimental outcomes demand.

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Target Background

Function(From Uniprot)

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Gene References into Functions

We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development. PMID:29186361
Plasma kallikrein (PLK)-dependent transforming growth factor-beta (TGF-beta) activation could be detected in isolated pancreatic stellate cells (PSCs) from chronic pancreatitis (CP) and pancreatic cancer. PMID:28187111
High molecular weight kinin functions as a cofactor for PK activation in the presence of nucleic acids in a manner consistent with classic models of contact activation. PMID:28124063
genetic polymorphism is associated with C1 Inhibitor deficiency angioedema age of onset in European families PMID:29130992
these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1 PMID:26566265
von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth. PMID:27581227
KLKB1 mRNA expression is a putative molecular biomarker in chronic lymphocytic leukemia. PMID:25891023
Genotyping these subjects revealed that the carriers of the minor alleles at the two loci- F12 and KLKB1 had a significant association with reduced levels of active plasma renin. PMID:26969407
2 genetic loci (kininogen 1 and kallikrein B) influencing key components of the RAAS, consistent with the close interrelation between the kallikrein-kinin system and the RAAS. PMID:25477429
PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID:25324000
Prekallikrein deficiency in a family is due to a new mutation (Arg541Gln) in exon 14. PMID:25075649
FXI may have a role in risk of ischemic stroke, but not myocardial infarct; FXII and prekallikrein may not have a role in either PMID:24977287
The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II. PMID:23672780
Investigate the relationship between circulating PK levels in children with metabolic syndrome and CVD risk factors because PK may be involved in the progression of the disease state. PMID:20725119
Case Report: Fresh frozen plasma transfusion before coronary artery bypass corrects prekallikrein deficiency. PMID:20135073
plasma kallikrein and FXIa activate pro-HGF in vitro PMID:12372819
the effect of kininogen degradation by human neutrophil elastase (HNE) on kinin generation by tissue and plasma kallikreins. PMID:12887060
prekallikrein preferential binding to endothelial cells contributes to their anticoagulant nature PMID:12944405
Crystallization and x-ray crystal structure determination have yielded the first three-dimensional views of the catalytic domain of plasma kallikrein PMID:16199530
Our findings suggested that common genetic variation in the KLKB1 gene might contribute to the risk of hypertension in the northern Han Chinese population. PMID:17318641
study demonstrates by immunohistochemistry that plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues PMID:17696780
three-dimensional structural data for prekallikrein (PK); interaction between PK and high-molecular-weight kininogen is mediated by two discrete surfaces formed by the PK A1, A2 and A4 domains with charge likely to be a critical component of the binding PMID:17922805
Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway PMID:18160576
Kallikrein is responsible for the cleavage of factor H. PMID:18413232
prekallikrein is an enzyme that can cleave high-molecular-weight kininogen to release bradykinin, and this reaction is inhibited by C1 inhibitor. PMID:19342086
NPY3-35 is a new peptide generated by plasma kallikrein PMID:19620246