ELANE

The ELANE gene encodes neutrophil elastase, a serine protease crucial for neutrophil function. Known as elastase, neutrophil expressed, this protein is primarily expressed in neutrophils and plays a pivotal role in inflammation and infection defense. It modifies cell and protein functions to combat infections by degrading extracellular matrix proteins. ELANE mutations are associated with cyclic neutropenia and severe congenital neutropenia, leading to recurrent infections and increased susceptibility to infections. Research into ELANE mutations has spurred drug development for neutropenia treatment, focusing on G-CSF therapy and hematopoietic stem cell transplantation.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELANE Recombinant Monoclonal Antibody(CSB-RA200985A0HU)

Validated Data

IHC image of CSB-RA200985A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Overlay histogram showing Jurkat cells stained with CSB-RA200985A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.

The following ELANE reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

ELANE Antibodies

ELANE Proteins

ELANE ELISA Kits

ELANE Antibodies

ELANE Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA007587LA01HU	ELANE Antibody	Human	ELISA, IHC, IF
CSB-PA007587LB01HU	ELANE Antibody, HRP conjugated	Human	ELISA
CSB-PA007587LC01HU	ELANE Antibody, FITC conjugated	Human
CSB-PA007587LD01HU	ELANE Antibody, Biotin conjugated	Human	ELISA
CSB-RA200985A0HU	ELANE Recombinant Monoclonal Antibody	Human	ELISA, IHC, FC

ELANE Proteins

ELANE Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-MP7944MOV	Recombinant Macaca fascicularis neutrophil elastase isoform X1 (Elane)	Mammalian cell

ELANE Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-MP7943RA	Recombinant Rat Elastase 2, neutrophil (Elane)	Mammalian cell

ELANE Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-EP007587HU	Recombinant Human Neutrophil elastase (ELANE)	E.coli
CSB-YP007587HU CSB-EP007587HU CSB-BP007587HU CSB-MP007587HU CSB-EP007587HU-B	Recombinant Human Neutrophil elastase (ELANE)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP007587HU	Recombinant Human Neutrophil elastase (ELANE)	Yeast
CSB-EP007587HU-B	Recombinant Human Neutrophil elastase (ELANE),Biotinylated	E.coli
CSB-EP007587HUe1	Recombinant Human Neutrophil elastase (ELANE)	E.coli
CSB-EP007587HUb0	Recombinant Human Neutrophil elastase (ELANE)	E.coli
CSB-EP007587HUc7	Recombinant Human Neutrophil elastase (ELANE)	E.coli
CSB-MP007587HU	Recombinant Human Neutrophil elastase (ELANE)	Mammalian cell
CSB-BP007587HU	Recombinant Human Neutrophil elastase (ELANE)	Baculovirus
CSB-MP007587HU1	Recombinant Human Neutrophil elastase (ELANE), partial	Mammalian cell
CSB-MP007587HU1r13	Recombinant Human Neutrophil elastase (ELANE), partial	Mammalian cell

ELANE Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP659740MO CSB-EP659740MO CSB-BP659740MO CSB-MP659740MO CSB-EP659740MO-B	Recombinant Mouse Neutrophil elastase (Elane)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP659740MO	Recombinant Mouse Neutrophil elastase (Elane)	Yeast
CSB-EP659740MO	Recombinant Mouse Neutrophil elastase (Elane)	E.coli