Abbreviation
Recombinant Human BSG protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Alternative Names
(CD147)(TCSF)(EMMPRIN)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-205aa
Target Protein Sequence
AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 2
Tag Info
C-terminal hFc1-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21.95-33.12 ng/ml.
Description
Basigin (CD147) drives tumor invasion and metastasis through its roles in matrix metalloproteinase induction and integrin-mediated adhesion, making it a critical target in cancer biology. This recombinant human BSG construct spans residues 22–205 of Isoform 2, covering the key extracellular immunoglobulin-like domains, and carries a C-terminal hFc1 tag that facilitates use in integrin-ligand binding studies, cell migration and invasion assays, and antibody development workflows. Functional ELISA confirms robust binding activity, with immobilized CD147 at 2 μg/ml engaging an anti-CD147 recombinant antibody at an EC50 of 21.95–33.12 ng/ml, providing a reliable basis for cell adhesion and spreading assays as well as tumor metastasis research. Mammalian cell expression preserves native glycosylation and folding essential for physiologically relevant protein–protein interactions, while purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg satisfy the criteria typically required for sensitive cell-based functional assays.
