Abbreviation
Recombinant Human CLDN9 protein-VLPs (Active)
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL.
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
1-217aa
Target Protein Sequence
MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length
Tag Info
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
CSB-MP005511HU is detected by Mouse anti-6*His monoclonal antibody.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL.
Description
Claudin-9 plays a critical role in tight junction integrity and has emerged as a target of interest in tumor metastasis research, where its dysregulation influences paracellular permeability and cancer cell invasion. This recombinant human CLDN9 is presented on virus-like particles (VLPs) to preserve native transmembrane topology and conformational epitopes—a key advantage for studying multi-pass membrane proteins that lose structural fidelity when solubilized. Functional ELISA confirms binding to an anti-CLDN6/9 recombinant antibody with an EC50 of 24.93–35.47 ng/mL, supporting use in antibody development and validation, integrin-ligand or selectin-ligand binding studies, and cell migration and invasion assays relevant to metastasis biology. The full-length construct (aa 1–217) with a C-terminal 10xHis tag, expressed in mammalian cells and carrying endotoxin levels below 1.0 EU/μg, provides a suitable basis for functional screening in cancer-focused immunological workflows.
